Found 9758 publications. Showing page 249 of 391:
2005
2007
2008
2024
2016
NILU og Urbanet Analyse har på oppdrag fra Miljødirektoratet utviklet modellen NERVE («Norwegian Emissions from Road
Vehicle Exhaust») for klimagassutslipp fra veitrafikken i norske kommuner. NERVE beregner klimagassutslipp fra
veitrafikken totalt innenfor hver kommune geografisk og for kommunens innbyggere, både som totalt utslipp og som en
utslippsfaktor (g/km). NERVE en en «bottom-up» modell som bygger på fire detaljerte datasett; 1) Veinettet ved alle
offentlige veier fra Nasjonal vegdatabank (NVDB), 2) trafikk på vei fra Regional Transport Model (RTM), 3)
kjørelengdestatistikken for norskregistrerte kjøretøy fra Statistisk Sentralbyrå Norge (SSB) og 4) utslippsfaktorer fra HBEFA(Hand Book of Emission FActors for Road Transport.
NILU
2018
NILU og Transportøkonomisk institutt (TØI) har på oppdrag fra Miljødirektoratet videreutviklet modellen NERVE («Norwegian Emissions from Road Vehicle Exhaust») for beregning av klimagassutslipp fra veitrafikken i norske kommuner. NERVE-modellen anvender de mest detaljerte datasettene for bilpark, utslippsfaktorer, trafikk og veier for spesifikke lokale forhold. Datasettene er kombinert i en datastruktur som gjør at resultat kan aggregeres på et lite eller et stort geografisk område. NERVE kan således betegnes som en «bottom-up»-utslippsmodell, fordi den er bygget opp «nedenfra» fra detaljerte datakilder. Denne rapporten presenterer metodikken og antagelsene bak beregningene med NERVE, og sammenligner resultat aggregert på nasjonalt nivå med annen tilgjengelig nasjonal statistikk.
NILU
2024
2016
2020
2006
2006
2015
New advanced models (NAMs) for risk assessment of bisphenol A alternatives
The safety of bisphenol A (BPA) due to its adverse effects on the immune system has led to an increasing concern and a significant regulatory shift. The European Food Safety Authority (EFSA) proposed a reduction in the tolerable daily intake (TDI) of BPA in food in their 2023 scientific opinion, highlighting the need for stricter regulations compared to their previous assessment in 2015. This regulatory action has spurred the production of BPA alternatives, raising concerns about their safety due to insufficient toxicological data. Addressing this gap is crucial for ensuring human and environmental health. In this project, multiple genotoxicity endpoints were applied for testing of two regulatory relevant BPA alternatives, bisphenol E (BPE) and bisphenol P (BPP), in different human models: 2D HepG2 liver cells, 3D liver spheroids and primary human peripheral blood lymphocytes. DNA strand breaks and oxidised base lesions were evaluated by the enzyme-modified version of the comet assay, while clastogenicity and aneugenicity were analysed by the in vitro micronucleus assay (OECD TG 487, 2016), together with cytotoxicity. Development of new advanced models (NAMs), as 3D spheroids, are essential for next-generation risk assessment (NGRA) in line with the 3R's to replace, reduce or refine animal experiments. In this aspect, validation and standardisation of NAMs are needed to reach regulatory readiness level and development of OECD Test Guidelines. Therefore, a standardisation and pre-validation of the advanced 3D liver spheroid model was performed by using multiple genotoxicity endpoints and by comparing the obtained results with standard genotoxicity models.
2024
Carcinogenic chemicals, or their metabolites, can be classified as genotoxic or non-genotoxic carcinogens (NGTxCs). Genotoxic compounds induce DNA damage, which can be detected by an established in vitro and in vivo battery of genotoxicity assays. For NGTxCs, DNA is not the primary target, and the possible modes of action (MoA) of NGTxCs are much more diverse than those of genotoxic compounds, and there is no specific in vitro assay for detecting NGTxCs. Therefore, the evaluation of the carcinogenic potential is still dependent on long-term studies in rodents. This 2-year bioassay, mainly applied for testing agrochemicals and pharmaceuticals, is time-consuming, costly and requires very high numbers of animals. More importantly, its relevance for human risk assessment is questionable due to the limited predictivity for human cancer risk, especially with regard to NGTxCs. Thus, there is an urgent need for a transition to new approach methodologies (NAMs), integrating human-relevant in vitro assays and in silico tools that better exploit the current knowledge of the multiple processes involved in carcinogenesis into a modern safety assessment toolbox. Here, we describe an integrative project that aims to use a variety of novel approaches to detect the carcinogenic potential of NGTxCs based on different mechanisms and pathways involved in carcinogenesis. The aim of this project is to contribute suitable assays for the safety assessment toolbox for an efficient and improved, internationally recognized hazard assessment of NGTxCs, and ultimately to contribute to reliable mechanism-based next-generation risk assessment for chemical carcinogens.
Frontiers Media S.A.
2023
Genotoxicity assessment is essential for ensuring chemical safety and mitigating risks to human health and the environment. Traditional methods, reliant on animal models, are time-consuming, costly, and raise ethical concerns. New Approach Methods (NAMs) offer innovative, cost-effective, and ethical alternatives, playing a pivotal role in both traditional and next-generation risk assessment (NGRA) by minimizing the need for animal testing, particularly in genotoxicity evaluations. However, the development of NAMs often overlooks the particular physicochemical properties of nanomaterials (NMs), which significantly influence their toxicological behaviour and can interfere with genotoxicity evaluation. This underscores an urgent need for the standardization and adaptation of NAMs to address nano- and advanced material-specific genotoxicity challenges. In this review, we summarize the challenges associated with genotoxicity testing of NMs and highlight the suitability of existing in vitro and in silico NAMs for NMs and advanced materials, enabling genotoxicity testing across various exposure routes and organ systems. Despite considerable progress, regulatory validation remains constrained by the absence of approved test guidelines and standardized protocols. To achieve regulatory acceptance, it is crucial to adapt NAMs to NM-specific exposure scenarios, refine test systems to better mimic human biology, develop tailored in vitro protocols, and ensure thorough characterisation of NMs both in pristine form and dispersed in culture medium. Collaborative efforts among scientists, regulators, industry, and advocacy groups are vital to improving the reliability and regulatory acceptance of NAMs. By addressing these challenges, NAMs have the potential to revolutionize genotoxicity risk assessment, advancing it towards a more sustainable, efficient and ethical framework.
2025
2014
Pergamon Press
2018
New brominated flame retardants in Arctic biota. Statlig program for forurensningsovervåking. Rapport 1070/2010. TA-2630/2010
2010