Found 10001 publications. Showing page 102 of 401:
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Transboundary particulate matter, photo-oxidants, acidifying and eutrophying components
Norwegian Meteorological Institute
2019
ICOS Norway – a carbon cycle infrastructure
The Integrated Carbon Observation System (ICOS) research infrastructure is aimed at quantifying and understanding the greenhouse gas balance of Europe and neighboring regions. ICOS-Norway brings together the leading
Norwegian institutes for greenhouse gas observations in the three Earth system domains atmosphere, ocean, and
terrestrial ecosystems, providing world-leading competence, which is integrated into one jointly funded and operated infrastructure. This provides Norway with a state-of-the-art research infrastructure embedded in European
and global efforts. Even though each Earth system domain was part of dedicated research infrastructures prior to
the establishment of ICOS-Norway, the greenhouse gas community in Norway was divided and there was minimal
collaboration across the Earth system domains. The overall goal of ICOS-Norway is to provide accurate and accessible data on, as well as integrated assessments of, the Norwegian carbon balance at regional scale, across the land,
ocean, and atmosphere. ICOS-Norway has thus led to an increased impact of environmental observing systems
in Norway and surrounding seas, easily seen through the number of publications and new proposals generated
as collaborative efforts. This poster presents the ICOS-Norway infrastructure, including plans for expansion and
long-term funding.
2019
The comet assay applied to HepG2 liver spheroids
In accordance with the 3 Rs to reduce in vivo testing, more advanced in vitro models, moving from 2D monolayer to 3D cultures, should be developed for prediction of human toxicity of industrial chemicals and environmental pollutants. In this study we compared cytotoxic and genotoxic responses induced by chemicals in 2D and 3D spheroidal cultures of the human liver cancer cell line HepG2.
HepG2 spheroids were prepared by hanging drop technology. Both 3D spheroids and 2D monolayer cultures were exposed to different chemicals (colchicine, chlorpromazine hydrochloride or methyl methanesulfonate) for geno- and cytotoxicity studies. Cytotoxicity was investigated by alamarBlue assay, flow cytometry and confocal imaging. DNA damage was investigated by the comet assay with and without Fpg enzyme for detection of DNA strand breaks and oxidized or alkylated base lesions.
The results from the cyto- and genotoxicity tests showed differences in sensitivity comparing the 2D and 3D HepG2 models. This study shows that human 3D spheroidal hepatocellular cultures can be successfully applied for genotoxicity testing by the comet assay and represent a promising advanced in vitro model for toxicity testing.
2019
2019
DNA double‐strand breaks in Arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic
High levels of organochlorine contaminants (OCs) have been found in arctic char (Salvelinus alpinus) from Lake Ellasjøen, Bjørnøya (Norwegian Arctic). The aim of the present study was to investigate the potential genotoxic effect of environmental organochlorine contaminant exposure in arctic char from Ellasjøen compared with arctic char from the low‐contaminated Lake Laksvatn nearby. Blood was analyzed using agarose gel electrophoresis and image data analysis to quantify the fraction of total DNA that migrated into the gel (DNA‐FTM) as a relative measure of DNA double‐strand breaks (DSBs). Analysis by GC‐MS of muscle samples showed an average 43 times higher concentration of ΣOCs in arctic char from Ellasjøen (n = 18) compared with Laksvatn char (n = 21). Char from Lake Ellasjøen had a much higher frequency of DSBs, as measured by DNA‐FTM, than char from Lake Laksvatn. Principal component analysis and multiple linear regressions show that there was a significant positive relationship between DSBs and levels of organochlorine contaminants in the char. In addition, DSBs were less frequent in reproductively mature char than in immature char. The results suggest that organochlorine contaminants are genotoxic to arctic char.
2019
2019
The Forum for Air Quality Modelling (FAIRMODE) is a European network to exchange experiences and competence on the use of air quality models in the context of the Ambient Air Quality Directives. Its purpose is to identify and promote the use of good practices for air quality modelling and to propose harmonized ways to assess the quality of model-based air quality applications by EU Member States. The recommendations in this document are part of FAIRMODE’s contribution to the on-going fitness check of the two EU Ambient Air Quality Directives (Directives 2008/50/EC and 2004/107/EC) initiated by the European Commission. This document provides technical recommendations where the scientific consensus within FAIRMODE indicates that robust conclusions can be drawn, and identifies follow up actions. These recommendations might potentially affect the work of Member States in case they may be requested to be implemented. They may also be relevant to the outcome and follow-up to the fitness check of the Air Quality Directives. Finally, they have implications for the work of the FAIRMODE network itself, and guide future technical discussions
Publications Office of the European Union
2019